PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Quantity	Each
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

Invitrogen™ Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Standard PCR
PCR Method	qPCR,Standard PCR
Exonuclease Activity	5' - 3'
Polymerase	Taq DNA Polymerase
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	No
Starting Material	DNA

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Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Thermostability	Not Applicable
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqMan

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Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	Maxima H Minus
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 20 kb
Reaction Format	Separate components
Reaction Speed	15 to 30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C to 55°C
Technique	Reverse Transcription
Starting Material	RNA

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Invitrogen™ Platinum™ SuperFi II PCR Master Mixes

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>300X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

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Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion U Hot Start DNA Polymerase
GC-Rich PCR Performance	Medium
Content And Storage	• Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl₂ • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C.
Fidelity (vs. Taq)	25X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
For Use With (Application)	Hot-start PCR

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion Plus DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>100X
Size (Final Product)	20 kb or less
Reaction Format	Ready to use master mix
Reaction Speed	15 to 30 sec./kb
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
InhibitorToleranceSimple	High
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	8 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	Applied Biosystems

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Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	AccuPrime Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	9X
Size (Final Product)	20 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	Built-In Hot Start
For Use With (Application)	Hot-start PCR,High-fidelity PCR
Detection Method	Primer-probe

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Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Pricing and Availability
Specifications

Product Type	Multiplex Kit
For Use With (Equipment)	3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type	DNA
Technique	SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line	SNaPshot
Detection Method	Primer-probe

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Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	ArrayScript™ UP
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	RNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Run Time	Standard
Product Line	RNA-to-C_T, SYBR

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Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Content And Storage	Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Genotyping
PCR Method	qPCR
Multiplex Capability	Up to 3-plex, Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Hot Start	Built-In Hot Start
Concentration	2X
For Use With (Application)	Hot-start PCR

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Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion Hot Start II High-Fidelity DNA Polymerase
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

PCR Supplies

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