Test
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).
Particle Size | 8.6μm |
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Ligand Type | Cross-linked agarose |
Content And Storage | 4° to 30° in 20% ethanol |
pH Range | 3 to 12 |
Column Type | Gel Filtration Column |
Material | Borosilicate glass |
Max. Pressure | 435 psi |
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.
Content And Storage | 2° to 8°C |
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Product Type | Calibration Kit |
For Use With (Equipment) | High-resolution media and prepacked column |
Quantity | Each |
For Use With (Application) | Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl |
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |
StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.
Height (Metric) | 25 mm |
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Column Type | Affinity Column |
For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™ |
The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.
DynaMag-Spin magnet is optimized for efficient magnetic separation of all types of Dynabeads (range 1–4.5 μm in diameter) in small sample volumes (1.5 mL) in microcentrifuge tubes.
Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix is an improved version of CaptureSelect C-tag Affinity Matrix with a significantly increased binding capacity, offered here in pre-packed format.
Shipping Condition | Room Temperature |
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Stationary Phase | Anti-C-tagXL |
Type | Pre-packed Column |
Content And Storage | Store in refrigerator (2°C to 8°C). |
Form | Liquid Suspension |
Product Line | CaptureSelect™ |
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.