Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BamH I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Invitrogen™ Anza™ 9 NdeI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme Cloning System
Enzyme	Nde I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	Anza™ 10X Red Buffer, Anza™ 10X Buffer
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	Anza™

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

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Thermo Scientific™ ATP Solution (100 mM)

Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

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Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.

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Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	EcoR I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Thermo Scientific™ TBE Buffer (Tris-borate-EDTA) (10X)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).

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Thermo Scientific™ FastDigest NdeI

The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nde I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest NheI

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nhe I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Dpn I
Methylation Sensitivity	Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	Blunt
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nco I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Hind III
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Not I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Xba I
Methylation Sensitivity	Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ Eco31I (BsaI) (10 U/μL)

The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Eco31I (BsaI)
Methylation Sensitivity	CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X Buffer G
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Restriction Enzymes

Restriction Enzymes

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