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Invitrogen™ Fast SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Fast SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Brand: Invitrogen™ 4402956

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Product Code. 10678255

1696.00 € / 100 reactions

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Ambion Fast SYBRGreen Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. The kit includes sufficient reagents for 100 reactions. The Fast SYBRGreen Cells-to-C_T Kit contains AmpliTaq™ Fast DNA Polymerase, UP, which allows instant hot start, thus minimizing non-specific product formation with fast real-time PCR.

Includes optimized reagents to work efficiently, right out of the box
Simple, effective Cells-to-C_T technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA
2-Step qRT-PCR

Extraordinary Value
The kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBRGreen.

Extraordinary Ease
The protocol begins with a simple 7-minute sample preparation procedure. First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; Dnase treatment can be performed concurrently. The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at -20°C for up to 5 months. Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix.

Extraordinary Performance
To demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the Fast SYBRGreen Cells-to-C_T Kit. The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells. In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits. Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms including those from Applied Biosystems, Roche, and Bio-Rad.

Accessory Product:
The SYBRGreen Cells-to-C_T Control Kit (Cat. No. 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-C_T reactions.

Order Info

Shipping Condition: Dry ice

Specifications

Content And Storage	Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature.
Polymerase	AmpliTaq Fast DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Label or Dye	SYBR Green
Product Line	Cells-to-CT™, SYBR™
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	M-MLV
For Use With (Application)	RT-PCR
Hot Start	Built-In Hot Start
Sample Type	Cells

Detection Method	SYBR
Reaction Speed	Fast
Green Features	Fewer resources used and less waste, Less hazardous
Passive Reference Dye	ROX (Pre-mixed)
Purification Time	5 min.
Quantity	100 reactions
Shipping Condition	Dry Ice
Concentration	20X
No. of Reactions	100 reactions

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Documents

Product Information

Manuals

Instruction Manual: Fast SYBR® Green Cells-to-CT™ Kit Version: 4403786 Rev C File size: 1.2 MB

Quick Reference Card: Fast SYBR® Green Cells-to-CT™ Kit (English ) Version: 10 Mar 2011 File size: 219.2 kB

Protocol: Power SYBR® Green Cells-to-CT™ Kit (English ) Version: 10 Mar 2011 File size: 1.2 MB

Protocol: Fast SYBR® Green Cells-to-CT™ Kit (English ) Version: 10 Mar 2011 File size: 1.2 MB

Product Information Sheet: SYBR Green Cells-to-CT™ Control Kit (English ) Version: 01 Mar 2011 File size: 117.6 kB

Instruction Manual: Fast SYBR® Green Cells-to-CT™ Kit Quick Reference Card Version: 4405659 Rev B File size: 219.2 kB

Specification Sheets

Brochures & Specifications: SYBR® Green Cells-to-CT™ Kits File size: 1.1 MB

Scientific Resources

Brochures

Green Fact Sheet: Ambion Cells-to-CT Kits - Green Fact Sheet File size: 431.6 kB

Certificates

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Lot Number

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number2852138	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4402956
Lot Number2927697	Certificate TypeCertificate of Analysis	Date27/01/2025	Catalog Number4402956
Lot Number3033237	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog Number4402956
Lot Number2747078	Certificate TypeCertificate of Analysis	Date21/06/2023	Catalog Number4402956
Lot Number01333633	Certificate TypeCertificate of Analysis	Date22/09/2022	Catalog Number4402956

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