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  5. Invitrogen™ FirstChoice™ RLM-RACE Kit
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Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Marque:  Invitrogen™ AM1700

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Code produit. 10554445

  • 1170.00 € / Each

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For Research Use Only. All usage must comply with product instructions.


Includes: Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5M EDTA should be stored at –20°C. Nuclease-free water may be stored at any temperature.

Requires : SuperTaq Thermostable Taq DNA Polymerase sold separately. For optimal amplification of fragments ≥1 kb, use SuperTaq-Plus.
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Description

Description

The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

  • From RNA to PCR product in less than a day
  • Yields single, specific product from rare transcripts
  • Selects 5' and/or 3' ends of true messages
  • Efficient—
  • Al enzymatic reactions are optimized to ensure detection of even rarest mRNA
  • Final product size: 7kb or less
  • Optimal reaction temperature: 42°C
  • Sample: RNA

  • Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages
  • Improvement to basic RACE protocol
  • Selects full-length mRNA—no rRNA, tRNA or degraded mRNA

Optimal reaction temperature: 42°C

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Informations sur la commande

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY
Spécification

Spécification

RLM-RACE Kit
The following components should be stored at –20°C:
  • Calf Intestine Alkaline Phosphatase
  • 10X CIP Buffer
  • Tobacco Acid Pyrophosphatase
  • 10X TAP Buffer, T4 RNA Ligase
  • 10X T4 RNA Ligase Buffer
  • 5' RACE Adapter
  • 3' RACE Adapter
  • 5' RACE Outer Primer
  • 5' RACE Inner Primer
  • 3' RACE Outer Primer
  • 3' RACE Inner Primer
  • Ammonium Acetate Stop Solution
  • M-MLV Reverse Transcriptase
  • dNTP Mix
  • 10X RT Buffer
  • 10X PCR Buffer
  • Random Decamers
  • RNase Inhibitor
  • Mouse Thymus RNA
  • 5' RACE Outer Control Primer
  • 5' RACE Inner Control Primer
  • 5' PCR Control Primer
  • 3' RACE Control Primer
Nuclease-free water may be stored at any temperature.
42°C
Dry Ice
Ambion™, FirstChoice™
1 Kit
Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature.
cDNA
M-MLV
Kit
cDNA Libraries & Library Construction
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For Research Use Only. Not for use in diagnostic procedures.