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Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Brand: Applied Biosystems™ 4472908

229.35 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

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Product Code. 13256519

426.00 € / 500 reactions

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For Research Use Only. All usage must comply with product instructions.

Includes: 2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components.

This item is not returnable. View return policy

Description

Specific - minimize primer-dimer and non-specific amplification
Reproducible and sensitive - consistent amplification across a wide dynamic range
Bright - contains SYBR™ GreenER™ dye for maximum brightness
Carry-over contamination control - contains heat-labile UDG
Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity:

SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:

SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA

Contains SYBR™ GreenER™ Dye and Heat-labile UDG:

SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility:

SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems

Order Info

Shipping Conditions: Wet Ice

Specifications

Content And Storage	The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Form	Liquid
GC-Rich PCR Performance	High
Polymerase	AmpliTaq DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOne™, Standard Mode, AB 7500, Fast Mode, AB 7500, Standard Mode, AB StepOne™, Fast Mode, AB StepOnePlus™, Standard Mode
Passive Reference Dye	ROX (Pre-mixed)
Product Type	Real Time PCR SYBR Master Mix
Quantity	1 x 5 mL
Sufficient For	100 Reactions of 20 μL
Concentration	2X
No. of Reactions	500 Reactions
Volume	50 mL

Detection Method	SYBR
Format	Vial
PCR Method	qPCR
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Product Line	SYBR™
Purity or Quality Grade	UP (Ultra Pure)
Shipping Condition	Wet Ice
For Use With (Application)	Gene Expression
Hot Start	Built-In Hot Start
Sample Type	DNA (Genomic), cDNA

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Documents

Scientific Resources

Brochures

SYBR® Select Master Mix Product Bulletin File size: 434.6 kB

Product Bulletin: SYBR® Select Master Mix File size: 434.6 kB

Product Information

Manuals

Product Insert: SYBR Select Master Mix Version: B File size: 132.8 kB

SYBR Select Master Mix (Quick Reference) Version: 4473368 C 8 May 13 File size: 119.5 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2773388	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4472908
Lot Number2804957	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4472908
Lot Number2804961	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4472908
Lot Number2825348	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4472908
Lot Number2844421	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4472908

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