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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

Brand: Applied Biosystems™ N8080234

523.60 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10117254

748.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

This item is not returnable. View return policy

Description

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.

Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)₁₆ included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

Specifications

Content And Storage	• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL) • RNase Inhibitor, 100 μL (20 U/μL) • dNTP Mixture, 1 mL (2.5 mM each dNTP) • Oligo (dT)₁₆, 100 μL (50 μM) • Random Hexamers, 100 μL (50 μM) • 10X RT Buffer, 1.5 mL • MgCl₂ Solution, 1.5 mL Store at –15 to –25°C.
Technique	Reverse Transcription
Quantity	200 reactions
Ribonuclease H Activity	Yes
For Use With (Application)	Real Time PCR (qPCR)
Final Product Type	First-Strand cDNA
Reaction Format	Separate components
Size (Final Product)	Up to 5 kb

Format	Kit
Optimal Reaction Temperature	42°C
Reverse Transcriptase	MultiScribe
Shipping Condition	Dry Ice
Concentration	50 U/μL
No. of Reactions	200 Reactions
Reagent Type	Reverse Transcription
Starting Material	RNA

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For Research Use Only. Not for use in diagnostic procedures.

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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

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