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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Brand: Applied Biosystems™ N8080234
448.80 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)
Description
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.
Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.
Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions
Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
Specifications
• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL) |
|
| Reverse Transcription | |
| MultiScribe | |
| Dry Ice | |
| 50 U/μL | |
| 200 Reactions | |
| Reverse Transcription | |
| RNA |
| Kit | |
| 42°C | |
| Yes | |
| Real Time PCR (qPCR) | |
| First-Strand cDNA | |
| Separate components | |
| Up to 5 kb |
For Research Use Only. Not for use in diagnostic procedures.