CD62L (L-Selectin) Monoclonal Antibody (OX85), eFluor™ 660, eBioscience™, Invitrogen™

Description

Description: The OX85 monoclonal antibody reacts with rat CD62L, which is also known as L-Selectin. CD62L is a 76-kDa member of the selectin family that is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. This cell surface receptor binds glycosylated, fucosylated, sulfated, and sialylated glycoproteins including CD34, GlyCAM-1, and MadCAM-1. These interactions mediate lymphocyte rolling on activated endothelium at sites of inflammation and homing to high endothelial venules of peripheral lymphoid tissues. Applications Reported: This OX85 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OX85 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.