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Invitrogen™ CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent

CellEvent™ Caspase-3/7 Green Ready Flow™ Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells.

Brand:  Invitrogen™ R37167

492.10 EUR valid until 2025-02-21
Use promo code "24115" to get your promotional price.

Product Code. 15945920

  • 534.00 € / 120 tests

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The product includes 2 reagents: the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Exceptionally bright green fluorescence upon binding to nucleic acids
• Rapid staining of apoptotic cells without wash steps

CellEvent Caspase-3/7 Green Ready Flow Reagent is a cell-permeant reagent that detects caspase 3/7 activity using flow cytometry. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

CellEvent Caspase-3/7 Green Ready Flow Reagent consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorescent signal having absorption/emission maxima of ∼502/530 nm. When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

120 assays
Caspase 3/7 Reagent
504/523
Room Temperature
CellEvent™ Ready Flow
Other Label(s) or Dye(s)
Dropper Bottle
4 dropper bottles, store at room temperature
Green
Nucleus
CellEvent™ Caspase 3/7 Green, SYTOX AADvanced Dead Cell Stain
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