missing translation for 'onlineSavingsMsg'
Learn More

Molecular Probes™ ChromaTide™ Texas Red™-12-dUTP

ChromaTide™ Texas Red™-12-dUTP

Brand:  Molecular Probes™ C7631

608.00 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.


Product Code. 10307122

  • 800.00 € / Each

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Molecular Probes™ ChromaTide™ dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide™ dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide™ Labeled Nucleotides Specifications:
  • Ex/Em of dye: Texas Red™-12-dUTP (595/615 nm)
  • Length of alkynylamino linker: 12 atoms

    Methods for Incorporating ChromaTide™ Nucleotides Into Probes
  • Nick translation
  • Random primer labeling
  • End-labeling with terminal deoxynucleotidyl transferase
  • Reverse transcription
  • PCR amplification

    See Methods for Enzymatic Incorporation of ChromaTide™ dUTPs for specific guidelines for each of these methods.

    Alexa Fluor™ and BODIPY™ Fluorescent Dyes Make Excellent Probes
    Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY™ and Alexa Fluor™ dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY™ dyes helps ensure minimal spectral overlap. The Alexa Fluor™ dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

    Long Linkers Improve Performance
    The ChromaTide™ dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

    For complete listing of our ChromaTide™ Reagents: Molecular Probes ChromaTide™ and aha labeled nucleotides—Table 8.5.
    For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

    For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
    TRUSTED_SUSTAINABILITY
  • Specifications

    Specifications

    Store in freezer (-5 to -30°C) and protect from light.
    Wet Ice
    Texas Red™
    ChromaTide™, Texas Red™
    Direct Labeling
    1 mM
    25 μL
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Promotions

    Promotions

    For Research Use Only. Not for use in diagnostic procedures.