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Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM)

Stain live or fixed cells with this far-red DNA stain.

Specifications

Cell Permeability Cell-permeant
Color Red
Content And Storage Store at 4°C.
Detection Method Fluorescence
For Use With (Application) Cell Imaging
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Products 3
Product Code Brand Description Target Quantity Price Quantity & Availability  
Product Code Brand Description Target Quantity Price Quantity & Availability  
11863865
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Thermo Scientific™
62251
DRAQ5™ Fluorescent Probe Solution (5 mM) 200 μL N/A
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11895005
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Thermo Scientific™
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DRAQ5™ Fluorescent Probe Solution (5 mM) 1 mL N/A
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Thermo Scientific™
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Cellomics DRAQ5 Fluorescent Probe 50 μL N/A
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Description

Description

Stain live or fixed cells with Thermo Scientific™ DRAQ5 Fluorescent Probe, a far-red DNA stain.

Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA, fluorescence microscopy and high-content imaging require a fluorescent label to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a fluorescent spectrum different than the other probes. The blue-fluorescent DNA-binding probes, Hoechst and DAPI are frequently used; however, these probes cannot be used when UV illumination is unavailable or other blue-emitting fluorescent probes are used. Therefore, nuclear probes that emit in a color other than blue is useful for cell identification and counting, and for determining nuclear morphology and DNA content.
  • Due to its far-red excitation and emission, the DRAQ5 Fluorescent Probe can be multiplexed with many other fluorophores and is ideal for cells expressing green fluorescent protein (GFP) fusion proteins
  • Compatible with many existing protocols across a wide range of instrumentation platforms
  • Emits in the far-red region, is lipophilic and crosses cell and nuclear membranes in live and fixed cells and tissues for rapid DNA staining
  • Stain is water-soluble, supplied ready-to-use and does not require RNase, cell lysis, or a washing step, making it compatible with automation
  • Because DNA staining is stoichiometric, the DRAQ5 Fluorescent Probe can be used for DNA content analysis in cell proliferation studies
Specifications

Specifications

Cell-permeant
Store at 4°C.
Cell Imaging
Fluorescent Probe Solution
681 nm
Liquid
> 99%
Nucleus, Cytosol, Cytoplasm
Red
Fluorescence
Fluorescence Microscope, High Content Analysis Instrument
Cell-Permeant
647 nm
Pierce™
Soluble at working concentrations in water and typical buffers
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Safety and Handling

Safety and Handling

Product Identifier
  • DRAQ5 Fluorescent Probe Solution (5 mM)

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.