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Invitrogen™ Dynabeads™ M-450 Tosylactivated

Dynabeads™ M-450 Tosylactivated

Brand:  Invitrogen™ 14013

Product Code. 15424693

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Description

Description

Dynabeads™ M-450 Tosylactivated are 4.5μm superparamagnetic beads containing surface tosyl groups. The beads covalently bind primary amino and sulfhydryl groups in antibodies to position them in the optimal orientation for cell-surface protein binding. Low background and covalent binding of the antibodies make these beads an excellent choice for cell separation applications when a downstream release from the beads is not required.

  • Quickly deplete cells or positively isolate target cells for downstream molecular analysis
  • Excellent for cell isolation procedures using your own unique antibody
  • Several antibodies can be coupled on the same beads for non-cell-based applications, such as cell activation

    Coupling procedure
    Covalent coupling is performed overnight by incubating the antibody of choice with the Dynabeads™ M-450 Tosylactivated beads. Optimal coupling occurs at high pH (8.5–9.5) and at 37°C. For pH-labile antibodies, coupling can be performed in an alternative buffer at pH 7.4.

    Not recommended for immunoprecipitation (IP)
    These 4.5μm Tosylactivated beads are not recommended for IP as they have a lower surface capacity per unit mass when compared to 1μm and 2.8μm Tosylactivated and Epoxy Dynabeads™.

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  • Find Dynabeads™ products for a whole range of applications.
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    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    2°C to 8°C
    Dynabeads™ M-450 Tosylactivated, 5mL, Suspension Format, Blood, PBMC, Tissue Digests Sample Type, Depletion, Positive Isolation Method, Used For Cell Isolation and Expansion, Protein Purification & Isolation
    Suspension
    2 x 107
    5 mL
    5mL Beads at a concentration of 4 x 10^8 beads/mL in distilled water
    Protein Biology, Protein Purification and Isolation, Cell Lysis and Fractionation, Cell Analysis, Cell Expansion, Cell Isolation, Organelle Isolation
    5mL beads at a concentration of 4 x 10^8 beads/mL in distilled water
    Research Grade
    Blood, PBMC, tissue digests
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    For Research Use Only. Not for use in diagnostic procedures.