Invitrogen™ E-Gel™ 48 Agarose Gels with SYBR™ Safe DNA Gel Stain, 2%

Description

Invitrogen E-Gel 48 high-throughput precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis. Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run.

Each gel contains 48 sample wells and 4 marker wells in 1% or 2% high-resolution agarose with a 3.2-cm run length. The various agarose percentages are used to separate DNA fragments of different sizes:
• 1% agarose: DNA fragments between 400 bp and 10 kb
• 2% agarose: DNA fragments between 50 bp and 3 kb

Each cassette is individually labeled with an EAN39 bar code that is recognized by most commercially available robotic bar code readers.

Features of E-Gel 48 gels include:
• High throughput analysis—contains 48 sample lanes and 4 marker lanes per gel
• Automation compatible—suitable for use with multi-channel pipettors and commonly used 8- and 12-pin liquid handling robots
• Scalable—resolve up to 192 sample in one run by combining up to four E-Base Mother and Daughter devices
• Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
• High performance—detect 2–3 ng of sample DNA with a separation time as short as ∼20 minutes

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Unlike ethidium bromide, the DNA stain most commonly incorporated into agarose gels, SYBR Safe DNA Gel Stain is a non-toxic, non-mutagenic DNA stain that is safer for the user, safer for the environment, and can save institutional overhead costs for disposal.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels.

Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not require any gel or buffer preparation or gel straining steps. Just load your samples and run.

Nucleic acid markers
For optimal DNA analysis on E-Gel 48 agarose gels we recommend the specially formulated E-Gel 96 High Range DNA Ladder (Cat. No. 12352019) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.


Running and imaging devices
High-throughput E-Gel agarose gels require the E-Base Electrophoresis Device for gel running.


Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The high-throughput E-Gel 96 and 48 agarose gels with SYBR Safe are available in 1% and 2% gel percentages.