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Merck Immobilon Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Brand:  Merck WBLUF0500

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Product Code. 12634645

  • 782.00 € / Each
Estimated Shipment: 05-12-2024
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Description

Description

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

  • Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

  • Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
  • Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
  • Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

  • Immobilon Classico premixed reagent for everyday detection needs
  • Immobilon Crescendo premixed reagent for detection in the picogram range
  • Immobilon Forte premixed reagent for detection in the femtogram range
TRUSTED_SUSTAINABILITY
Specifications

Specifications

HRP Substrate
500 mL
18°C to 24°C
Dot/Slot Blot
∼400 fg
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