missing translation for 'onlineSavingsMsg'
Learn More

Gibco™ Essential 6™ Medium

Essential 6™ Medium

Brand:  Gibco™ A1516401

249.40 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.


Product Code. 15148645

  • 290.00 € / 500mL

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Essential 6 Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and the spontaneous or directed differentiation of human pluripotent stem cells (PSCs). In addition, Essential 6 Medium can be used as a base for custom media for the culture of PSCs. The formulation is based on a medium originally developed by Guokai Chen et. al. (1) in the laboratory of James Thomson and published as “E6.” With only six essential components, Essential 6 Medium helps minimize variability. To complete your workflow with a matching reduced-variability PSC culture medium developed by the same lab, use Essential 8 Medium.

Essential 6 Medium enables:
  • Differentiation—does not contain bFGF, which inhibits differentiation
  • Reprogramming—does not contain TGFβ, which has a negative effect on reprogramming efficiency
  • Flexibility—provides a flexible format where the levels of TGFβ and bFGF can be adjusted and additional components can be added to match a given application
Differentiation
Unlike other media used in PSC culture, Essential 6 Medium does not contain bFGF or TGFβ. As such, Essential 6 Medium can support the formation of embryoid bodies. It has also been used as a base for the directed differentiation of various cell types in the endodermal, mesodermal, and ectodermal lineages (2), including motor neurons (3).

Reprogramming
Essential 6 Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (4) and CytoTune (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.

Flexibility
Essential 6 Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFβ. This provides a basal medium that will maximize cell health and pluripotency while allowing levels of TGFβ and bFGF to be adjusted and additional components to be added to match a given application.

Commercialized in partnership with Cellular Dynamics International.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store at 2 to 8°C and protect from light.
Xeno-free
Liquid
Serum-free
cGMP for medical devices, 21 CFR Part 820 and ISO 13485
500 mL
Human
Pluripotent Stem Cells
Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
Stem Cell Media
Phenol Red
Essential 6™
Ambient
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.