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Gibco™ Expi293™ GnTI- Expression System Kit

Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells

Brand:  Gibco™ A39250

2176.00 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 16366395

  • 2565.00 € / 1 set
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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.

Features:

  • Homogeneous N-glycosylation of expressed proteins
  • Equivalent protein yields to parental Expi293F cells - up to 1 g/L
  • Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
  • Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
  • High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Components included:

  • 1 vial of frozen Expi293F GnTI- cells
  • 1 L of Expi293 Expression Medium
  • 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
  • 100 mL Opti-Plex Complexation Buffer
  • 1 PNGase F Glycan Cleavage kit
  • pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 GnTI- Expression System are also available for individual purchase

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Transfection
• Expi293F GnTI- Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• PNGase F Glycan Cleavage Kit, store at -20°C
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C
Expi293F™
Protein Production
1 kit
Approved for shipment at Room Temperature or on Wet or Dry Ice
Expi293™
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For Research Use Only. Not for use in diagnostic procedures.