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Invitrogen™ eBioscience™ eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit
Designed to rapidly identify cytokine producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples.
Brand: Invitrogen™ 8822-6854-41
Alert:
Contains Sodium azide and formaldehyde
Includes:
- eZKine CD8 Activation 1 Cocktail : 25 tests. This cocktail contains the following antibodies:
- Anti-Human CD3 (SK7) APC
- Anti-Human CD8 (SK1) PerCP-eFluor 710
- Anti-Human CD69 (FN50) PE
- Anti-Human IFN gamma (4S.B3) FITC
- eZKine Isotype Control Cocktail D : 25 tests. This cocktail contains the following antibodies:
- Anti-Human CD3 (SK7) APC
- Anti-Human CD8 (SK1) PerCP-eFluor 710
- Mouse IgG1 K Isotype Control PE
- Mouse IgG1 K Isotype Control FITC
- eZKine Fix/Lyse Concentrate (4X)
- eZKine Fix/Lyse Diluent
- Permeabilization Buffer (10X)
Description
- Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization.
- Samples are then ready to stain with the CD8 Activation 1 cocktail and concentration-matched Isotype Control cocktail.
Specifications
| Human | |
| CD3, CD69, CD8, IFN gamma | |
| Intracellular Staining Followed by Flow Cytometric Analysis | |
| 2°C to 8°C. Do not freeze. Light sensitive material. |