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Thermo Scientific™ GeneJET Plasmid Miniprep Kit
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
Brand: Thermo Scientific™ K0502
This item is not returnable.
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Description
GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.
Highlights
• Efficient—high yields of up to 20 μg of high quality plasmid DNA
• Fast—procedure takes less than 14 minutes
• Convenient—no phenol/chloroform extraction or alcohol precipitation required
• Pure—purified DNA is immediately ready to use
Applications
• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation
Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol
Specifications
| Spin Column | |
| Silica Column | |
| Mini | |
| Compatible (Vacuum manifold) | |
| GeneJET | |
| 50 Preps | |
| <14 min |
| Spin Column | |
| Bacteria | |
| PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Plasmid MiniPrep Kit | |
| Plasmid DNA |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- GeneJET Plasmid Miniprep Kit
Signal Word
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Acute toxicity Category 4
Hazard Statement
- H319-Causes serious eye irritation.
- H315-Causes skin irritation.
- H302-Harmful if swallowed.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P264-Wash thoroughly after handling.
- P373-DO NOT fight fire when fire reaches explosives.
Supplemental information
- MIXTURE LIST-Contains : Sodium lauryl sulfate, Sodium hydroxide,Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.