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Fisher BioReagents™ SurePrep™ Water RNA/DNA Purification Kit
Provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from all microorganisms found in environmental water samples, including bacteria, fungi, and algae
Brand: Fisher BioReagents™ BP2814-25
Includes: Lysis solution (15mL); Enzyme incubation buffer (4mL); Nucleic acid elution buffer (4mL); Nucleic acid wash solution (11mL); Spin columns (25); Collection tubes (25); Elution tubes, 1.7mL (25); Bead tubes (25); Filter column, 0.45μm (25)
Description
- Suitable for use with small and large samples of water from a variety of sources, including ocean, pond, drinking water, and wastewater
- Optimized column, solution components, and user friendly protocol are provided for simultaneous isolation and purification of DNA and RNA
- All RNA species are isolated, in size ranges from large mRNA and ribosomal RNA to microRNA and siRNA
- Filter technology is used for the collection of microorganisms from water samples
- “Bead-beating” procedure helps break open the cells and allows for efficient lysis without the use of chemicals
- The same spin column can be used to simultaneously isolate both DNA and RNA or one nucleic acid, based on preference
- No additional step to concentrate RNA and DNA prior to isolation
- Rapid purification process is completed in 45 minutes
- No special equipment or hazardous chemicals are required
- High quality and high integrity DNA and RNA can be used for numerous downstream applications, including PCR, RT-PCR, QRT-PCR, Southern blotting, and Northern blotting
Specifications
| Spin Column, Filter Column | |
| PCR, RT-PCR, QRT-PCR, southern blotting and northern blotting | |
| Lysis solution 15mL, Enzyme incubation Buffer 4mL, Nucleic Acid Elution Buffer 4mL, Nucleic Acid wash solution 11mL, spin columns 25, collection tubes 25, 25 elution tubes, 1.7mL, bead tubes 25, 25 filter column, 0.45μm | |
| 25 Preps |