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Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit

Fluo-4 Direct™ Calcium Assay Kit

Brand:  Molecular Probes™ F10471

446.88 EUR valid until 2025-04-18
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Product Code. 10297783

  • 594.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

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Description

Description

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple addition only format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Green
Fluorescence
Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Fluorescent Dye-Based
Powder
Molecular Probes™
Room Temperature
  • 10 × 10 ml Fluo-4 Direct™ Calcium Assay Reagent (Component A)
  • 2 × 77 mg Probenecid (Component B)
  • 200 ml Fluo-4 Direct™ Calcium Assay Buffer (Component C)
  • Store at ≤-20°C. Dessicate and protect from light.
Calcium Assay
Cell-based calcium flux
Visible
Chemical
10 x 10 mL
Nucleus, Organelles, Cytoplasm
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Product Information

Manuals

Fluo-4 Direct Calcium Assay Kits Version: 04-03-2009 File size: 195.7 kB
Manual: Fluo4 Direct Calcium Assay Kits (Japanese) Version: Fluo4 Direct Calcium Assay Kits_J1_3Apr2009.doc File size: 428.4 kB
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number3071402 Certificate TypeCertificate of Analysis Date22/11/2024 Catalog NumberF10471
Lot Number2970998 Certificate TypeCertificate of Analysis Date08/07/2024 Catalog NumberF10471
Lot Number2916312 Certificate TypeCertificate of Analysis Date04/06/2024 Catalog NumberF10471
Lot Number2884589 Certificate TypeCertificate of Analysis Date11/04/2024 Catalog NumberF10471
Lot Number2820948 Certificate TypeCertificate of Analysis Date29/01/2024 Catalog NumberF10471
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For Research Use Only. Not for use in diagnostic procedures.