Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Description

• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
• Assay sensitivity is 100- to 1000-fold better than with either isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
• Highly sensitive assay with wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
• Assay can be completed in about one hour, providing fast assay turnaround

Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Ready-to-Use
The Galacto-Light Plus system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.

Specifications

• Assay Type: β-Gal (lacZ) Assay
• Compatible Cells: Mammalian Cells
• Detection Method: Chemiluminescent
• For Use With Equipment: Luminometer (Microplate)
• Form: Liquid
• Format: 96-well plate
• High Throughput Compatibility: High Throughput-Compatible
• Readout: End Point
• Sample Type (Specific): Cell Cultures
• Substrate: Galacton-Plus
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase