Learn More
Invitrogen™ Precision gRNA Synthesis Kit
Description
Includes
BOX 1
- 5X TranscriptAidTM Reaction Buffer
- DNase I, RNase-free (1 U/μL)
- Nuclease-free water
- NTP mix (100 mM each of ATP, GTP, CTP,
- UTP in Tris buffer)
- Control gRNA forward and reverse primers (10 μM mix) 2
BOX 2
- Binding Buffer
- Wash Buffer 1 (concentrated)3
- Wash Buffer 2 (concentrated)4
- Nuclease-free water
- Gene JETTM RNA Purification Micro Column & Collection Tubes
- Elution Tubes, 1.5 mL
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short one-pot PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.
Features of the GeneArt Precision gRNA Synthesis Kit include:
- Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
- High yield (>10 ug) and concentration (>200 ng/uL) of gRNA
For research use only. Not for use in diagnostic procedures.
Certifications
CoA
Recommended Storage
gRNA Prep Kit, store at -5 to -30°C and gRNA Cleanup Kit, store at room temperature
Specifications
Specifications
| Concentration | 200 ng/μL |
| Content And Storage | • gRNA Prep Kit, store at -5°C to -30°C • - gRNA Cleanup Kit, store at room temperature (For better long-term performance, store purification columns at 2°C to 8°C) |
| Form | Liquid |
| Format | Kit |
| Reaction Speed | Fast |
| Technique | CRISPR-Cas9 |
| Product Type | gRNA Synthesis Kit |
| Promoter | T7 |
| Quantity | 25 reactions |
| Shipping Condition | Approved for shipment at Room Temperature or on Wet or Dry Ice |
| Show More |
Safety and Handling
- GENEART GRNA KIT
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 1 B
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H412-Harmful to aquatic life with long lasting effects.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Thiocyanic acid, Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.