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Invitrogen™ Precision gRNA Synthesis Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.
Brand: Invitrogen™ A29377
Alert:
For research use only. Not for use in diagnostic procedures.
Includes:
BOX 1
- 5X TranscriptAidTM Reaction Buffer
- DNase I, RNase-free (1 U/μL)
- Nuclease-free water
- NTP mix (100 mM each of ATP, GTP, CTP,
- UTP in Tris buffer)
- Control gRNA forward and reverse primers (10 μM mix) 2
BOX 2
- Binding Buffer
- Wash Buffer 1 (concentrated)3
- Wash Buffer 2 (concentrated)4
- Nuclease-free water
- Gene JETTM RNA Purification Micro Column & Collection Tubes
- Elution Tubes, 1.5 mL
Description
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short ‘one-pot’ PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.Features of the GeneArt Precision gRNA Synthesis Kit include:
- Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
- High yield (>10 ug) and concentration (>200 ng/uL) of gRNA
Certifications
CoA
Recommended Storage
gRNA Prep Kit, store at -5 to -30°C and gRNA Cleanup Kit, store at room temperature
Specifications
| • gRNA Prep Kit, store at -5° to -30°C • gRNA Cleanup Kit, store at room temperature |
|
| Kit | |
| CRISPR-Cas9 | |
| T7 | |
| 25 Reactions | |
| 200 ng/μL | |
| 25 Reactions |
| Liquid | |
| Fast | |
| gRNA Synthesis Kit | |
| Approved for shipment at Room Temperature or on Wet or Dry Ice | |
| >10 μg at >200 ng/μL of gRNA | |
| RNA | |
| Oligos (DNA) |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- GENEART GRNA KIT
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 1 B
- Skin corrosion/irritation Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Thiocyanic acid, Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.