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Gibco™ Basal Medium Eagle (BME)
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Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.

Brand:  Gibco™ 21010046

Product Code. 15470604

  • 23.80 € / 500mL

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For Research Use Only. All usage must comply with product instructions.


Requires : Supplementation
This item is not returnable. View return policy

Description

Description

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

This BME is manufactured as follows:

  • With: Phenol Red
  • Without: L-glutamine, HEPES

The complete formulation is available.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Animal Origin-free
Liquid
Sterile-filtered
No Glutamine, No HEPES, No Sodium Pyruvate
Gibco™
Room Temperature
HeLa, mouse fibroblasts, WI-38 and MRC-5
1 X
Basal Medium Eagle (BME)
Low Glucose, Phenol Red
cGMP-compliant under the ISO 13485 standard
500 mL
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.