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Gibco™ Basal Medium Eagle (BME)
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.
Brand: Gibco™ 21010046
Description
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.
This BME is manufactured as follows:
- With: Phenol Red
- Without: L-glutamine, HEPES
The complete formulation is available.
BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.
BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Animal Origin-free | |
| Liquid | |
| Sterile-filtered | |
| No Glutamine, No HEPES, No Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
| HeLa, mouse fibroblasts, WI-38 and MRC-5 | |
| 1 X | |
| Basal Medium Eagle (BME) | |
| Low Glucose, Phenol Red | |
| Sustainable packaging | |
| Gibco™ | |
| Room Temperature |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.