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Gibco™ DMEM, powder, high glucose
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.
Brand: Gibco™ 12100061
Description
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: High Glucose, L-glutamine, Phenol Red
Without: Sodium Pyruvate, HEPES, Sodium Bicarbonate
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
|
| Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells | |
| Powder | |
| Standard Serum Supplementation | |
| High Glucose, Glutamine, Phenol Red | |
| 4500 mg/L | |
| Gibco™ | |
| Room Temperature |
| HeLa, 293, Cos-7, and PC-12 | |
| Animal Origin-free | |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate | |
| cGMP-compliant under the ISO 13485 standard | |
| 10 L |
In vitro diagnostic