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Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.
54.00 €
Specifications
| Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|---|---|
| Cell Line | CHO, COS-7, and rat prostate epithelial cells |
| Cell Type | Primary Rat Astrocytes |
| Classification | Animal Origin-free |
| Concentration | 1 X |
Requires: Supplementation
Description
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
With: GlutaMAX™, Phenol Red
The complete formulation is available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Gibco™ F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Rat Astrocytes | |
| 1 X | |
| Ham's F-12 Nutrient Mixture | |
| GlutaMAX, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| Room Temperature |
| CHO, COS-7, and rat prostate epithelial cells | |
| Animal Origin-free | |
| Liquid | |
| Sterile-filtered | |
| No HEPES | |
| Gibco™, GlutaMAX™ |
Safety and Handling
missing translation for 'shelfLife' : 12 Months From Date of Manufacture
For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
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