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  7. Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
  8. 10704217

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Brand:  Applied Biosystems™ 4387406

209.30 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10704217

  • 299.00 € / Each

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For Research Use Only. All usage must comply with product instructions.


Includes:

50 Reaction Kit: 1 x 50μL of 20X Enzyme mix (MuLV and RNase inhibitor protein); 1 x 500μL of 2X RT Buffer Mix (includes dNTPs, random octamers, and oligo dT-16)

This item is not returnable. View return policy

Description

Description

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 20X Enzyme mix (1 x 50 μL)
• 2X RT Buffer Mix (1 x 500 μL)

Store at –15 to –25°C.
Buffer Mix may be stored at 2–8°C for up to 6 months.
30 to 60 min.
37°C
MultiScribe
Dry Ice
First-Strand cDNA
Premixed Components
Up to 7 kb
Kit
Reverse Transcription
50 reactions
Yes
Real Time PCR (qPCR), RT-PCR
50 Reactions
Reverse Transcription
RNA
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For Research Use Only. Not for use in diagnostic procedures.