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  6. Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
  7. 11330292

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Brand:  Cytiva 28-9356-04


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Regulatory: This product contains a substance listed in Annex XIV to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 11330292

  • 865.00 € / Each
Estimated Shipment: 10-12-2024
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Description

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Each
25 to 75 μm
120 mL
1.6cm
23.62 in.
Polypropylene
3 to 11
Polysaccharides, macromolecules with extended structures and even small plasmids
<0.5 mL/min.
Low to medium pressure AKTA™ systems or pumped chromatography systems
Sephacryl HR Pre-packed Gel Filtration Column
Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
60cm
1.5 bar [0.15 MPa] (22 psi)
Allyl Dextran and N,N'-methylenebisacrylamide
4 to 30°C, 20% Ethanol
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Safety and Handling

Safety and Handling

Product Identifier
  • HiPrep 16/60 Sephacryl S-400 HR

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol

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