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Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids
Brand: Cytiva 28-9356-06
This item is not returnable.
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Description
Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).
- Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
- Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
- Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
- Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)
Media
- Average particle size: 50μm
- Matrix: Allyl dextran and N, N'-methylenebisacrylamide
- pH Stability working range: 3 to 11
- pH Stability cleaning range: 2 to 13
- Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
- Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
- Material (bed support): Polymethyl-Methacrylate
HiPrep 16/60 Sephacryl S-400 HR
- Media:
- Fractionation [Mr] globular proteins: 2 x 104-8 x 106
- Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
- Fractionation range [Mr] DNA exclusion limit: 271bp
- Fractionation [Mr] Dextrans: 1 x 104-2 x 106
- Media:
- Exclusion limit for Dextrans: 100 x 106
- Fractionation range [Mr] DNA exclusion limit: 1078bp
- Fractionation [Mr] Dextrans: 4 x 104-2 x 107
Specifications
| Each | |
| 25 to 75 μm | |
| 120 mL | |
| 1.6cm | |
| 23.62 in. | |
| Polypropylene | |
| 3 to 11 | |
| Polysaccharides, macromolecules with extended structures and even small plasmids |
| <1.3 mL/min. | |
| Low to medium pressure AKTA™ systems or pumped chromatography systems | |
| Sephacryl HR Pre-packed Gel Filtration Column | |
| Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids | |
| 60cm | |
| 1.5 bar [0.15 MPa] (22 psi) | |
| Allyl Dextran and N,N'-methylenebisacrylamide | |
| 4°C to 30°C, 20% Ethanol |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HiPrep 16/60 Sephacryl S-500 HR
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : ethanol