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Cytiva His SpinTrap™ Spin Columns
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.
Brand: Cytiva 28-4013-53
This item is not returnable.
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Description
- Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
- One run in standard microcentrifuge takes approximatedly 10 min.
- Yields up to 750μg purified protein per column
- Allows direct purification of both clarified and unclarifed cell lysates
- Also useful for screening large numbers of small-scale bacterial lysates
- Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
Medium Characteristics:
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Stable (long-term) in the pH range 3-12
- Average particle size: 34μm
Order Info
Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)
Specifications
| 100 μL | |
| 3 to 12 | |
| 4°C to 30°C | |
| Spin Column Kit |
| His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) | |
| 6% Highly Cross-linked Agarose | |
| 1 Pack His SpinTrap Spin Columns (50 x 100 μL) | |
| 600 μl |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Warning
Hazard Category
- Skin sensitiser Category 1
Hazard Statement
- H317-May cause an allergic skin reaction.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : nicke, reaction massof:5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2Hisothiazol-3-one