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Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Brand: Cytiva 17-0409-03
This item is not returnable.
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Description
- Uses different kinds of metal ions
- Binding capacity ∽12mg histidine-tagged protein/mL medium
- Metal ion capacity ∽23μmol Cu2+/mL medium
- Prepacked with Chelating Sepharose High Performance media
- Iminoacetic acid coupled to a 6% agarose matrix by ether linkage
Specifications
6M Guanidine Hydrochloride, 8M Urea | |
5mL | |
16mm | |
0.98 in. | |
<20 mL/min. | |
Iminodiacetic Acid | |
3 to 13 | |
6% Highly Cross-linked Agarose | |
Affinity Column |
HiTrap chelating HP column | |
0.62 in. | |
5 x 5mL | |
25mm | |
Polypropylene | |
3 bar | |
34μm | |
4° to 30°C | |
IMAC and subsequent purification of Polyhistidine tagged Proteins |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HiTrap Chelating HP 5 ml, 5 x 5 ml
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol
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