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Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Brand: Cytiva 17-0409-05
This item is not returnable.
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Description
- Uses different kinds of metal ions
- Binding capacity ∽12mg histidine-tagged protein/mL medium
- Metal ion capacity ∽23μmol Cu2+/mL medium
- Prepacked with Chelating Sepharose High Performance media
- Iminoacetic acid coupled to a 6% agarose matrix by ether linkage
Specifications
6M Guanidine Hydrochloride, 8M Urea | |
5mL | |
0.98 in. | |
<20 mL/min. | |
Iminodiacetic Acid | |
3 to 13 | |
6% Highly Cross-linked Agarose | |
Affinity Column |
HiTrap chelating HP column | |
100 x 5 mL | |
25mm | |
Iminiodiacetic acid chelating group | |
3 bar | |
34μm | |
4° to 30°C | |
IMAC and subsequent purification of Polyhistidine tagged Proteins |
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