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Cytiva HiTrap™ IMAC Columns
For optimizing purification of histidine-tagged proteins when Ni +2 is not the best choice of metal ion
Brand: Cytiva 17-0921-02
Includes: Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems
Description
- Conveniently charge with your metal of choice
- Can be used with a syringe, peristaltic pump, or liquid chromatography system
- Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
- High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
- Stable in the presence of all commonly used buffers
- Available in 1mL and 5mL sizes
Specifications
1 mL | |
<4 mL/min. | |
Prepacked with IMAC Sepharose 6 Fast flow media | |
Polypropylene | |
3 to 12 | |
HiTrap IMAC Fast Flow Column | |
5 x 1 mL |
0.27 in. | |
7 mm | |
Syringe, peristaltic pump or liquid chromato graphy system | |
42 psi | |
90 μm | |
4°C to 30°C | |
For optimizing purification of His-tagged Proteins |