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Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
Brand: Cytiva 28-9537-66
This item is not returnable.
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Description
Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
- Prepacked with cobalt-based chromatography media
- Binding capacity approximately 7mg MBP
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
- 1 and 5mL columns
Media
- Ligand: TALON™ Superflow Cobalt-IMAC
- Matrix: 6% cross-linked agarose
- Average particle size: 60 to 160μm
- pH stability working range: 3 to 12
1mL columns
- Column I.D.: 7mm
- Flow velocity: 1 to 4mL/min.
5mL columns
- Column I.D.: 16
- Flow velocity: 5 to 20mL/min.
Specifications
| 5 x 1 mL | |
| 4°C to 8°C | |
| 0.27 in. | |
| 0.7 cm | |
| 60 to 160 μm | |
| 3 bar | |
| 3 to 12 | |
| Talon Superflow Cobalt-IMAC |
| For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH | |
| Up to 20 mg Histidine-tagged Protein1 | |
| HiTrap Talon crude prepacked Column | |
| 1 to 4 mL/min | |
| Polypropylene | |
| 6% Cross-Linked Agarose | |
| 20% Ethanol | |
| 5 x 1 mL |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- EUH208-Contains cobalt. May produce an allergic reaction.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Ethanol, Methanol, Cobalt