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Proteintech™ Human GOLM1 ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous GOLM1

Brand:  Proteintech™ KE00111-96T


Product Code. 16891516

  • 269.00 € / 96 tests

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Description

Description

  • Recognizes human GOLM1
  • GOLM1 (GP73, GOLPH2) is a resident Golgi Type II membrane protein
    • Predominantly expressed in epithelial cells of many human tissues
    • Traffics through endosomes and secreted into circulation
    • Expression is upregulated in tumors and could be promising serum marker for hepatocellular carcinoma

Process:

  • Antibody specific for GOLM1 pre-coated onto microwells
  • GOLM1 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for GOLM1 is added to detect captured GOLM1 protein
  • For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

BC001740
0.01 ng/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
3.5 hr.
0.156 to 10 ng/mL
ISO 13485
Coated ELISA Kit
DAB
51280
Solid
<10%
-20°C to +4°C
Q8NBJ4
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