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  5. Invitrogen™ eBioscience™ Human HSP27 Platinum ELISA Kit

Invitrogen™ eBioscience™ Human HSP27 Platinum ELISA Kit

Product Code. 15561607
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Quantity:
10 x 96 Tests
2 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
96 tests
Pack of 10
Pack of 2
Pack of 5
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Product Code. 15561607

Brand: Invitrogen™ BMS2086TEN

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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human HSP27
Biotin-Conjugate anti-human HSP27 monoclonal antibody

For the quantitative detection of human HSP27.

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P04792
Assay Range 15.6-1000 pg/mL
Assay Sensitivity 10.9 pg/mL
Content And Storage Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human HSP27
Biotin-Conjugate anti-human HSP27 monoclonal antibody
Streptavidin-HRP
Human HSP27 Standard lyophilized, 2 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 27kDa; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 1; heat shock protein 27 kDa beta-1; heat shock protein B1; heat shock protein beta-1; heat shock protein family B (small) member 1; heat shock protein, 25 kDa; heat-shock protein; HEL-S-102; HMN2B; HS; HS.76067; HSP; HSP 25; HSP 27; Hsp25; HSP27; HSP28; HSPB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp2527kDa; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 1; heat shock protein 27 kDa beta-1; heat shock protein B1; heat shock protein beta-1; heat shock protein family B (small) member 1; heat shock protein, 25 kDa; heat-shock protein; HEL-S-102; HMN2B; HS; HS.76067; HSP; HSP 25; HSP 27; Hsp25; HSP27; HSP28; HSPB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25; CMT2F; HEL-S-102; HMN2B; HS.76067; HSP27; HSP28; Hsp25; SRP27
Gene ID (Entrez) 3315
Gene Symbol HSPB1
Interassay CV 4.3%
Intraassay CV 5.8%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
Label or Dye Biotin
Quantity 10 x 96 Tests
Regulatory Status RUO
Storage Requirements 2-8°C
Synonym Heat shock protein 27, heat shock protein beta-1, germ cell-dependent phosphoprotein-1
Target HSP27
Test Time 1 hr 20 min
Total Assay Time 3 hr 30 min
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