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Invitrogen™ IL-6 Human ELISA Kit
Measure levels of human interleukin-6 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.
Brand: Invitrogen™ EH2IL6
642.20 EUR valid until 2024-12-31
Use promo code "21957" to get your promotional price.
This item is not returnable.
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Description
Assay IL-6 (human interleukin-6) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-6 ELISA Kits.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
Specifications
| P05231 | |
| <1 pg/mL | |
| Biotin | |
| ELISA Kit | |
| No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6 | |
| Human | |
| 3569 | |
| <10% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Streptavidin-HRP Concentrate, 75µL | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 96 Tests | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL | |
| Human | |
| 3 hr. |
| 10.24-400 pg/mL | |
| Human IL-6 ELISA Kit | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| Plasma, Serum, Supernatant, Urine | |
| 96 microplate wells | |
| Colorimetric Microplate Reader | |
| BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6 | |
| <10% | |
| HRP | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |