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Molecular Probes™ BacLight™ Bacterial Membrane Potential Kit
BacLight™ Bacterial Membrane Potential Kit
Brand: Molecular Probes™ B34950
This item is not returnable.
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Description
The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.
Specifications
| Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light. | |
| Membrane Potential Assay | |
| Bacterial Membrane Potential Kit | |
| 482⁄497 | |
| BacLight™ | |
| Wet Ice |
| Fluorescence | |
| Flow Cytometer | |
| Membrane Potential Probes | |
| Tube(s) | |
| 1 kit |
For Research Use Only. Not for use in diagnostic procedures.