The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris.
Brand: Invitrogen™ K171001
The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.
| Ampicillin (AmpR) | |
| The Original Pichia Expression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5' AOX1, 3' AOX1, and α-factor sequencing primers. Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored., Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months |
|
| AOX1 | |
| Kit | |
| pPIC | |
| Cell-Based Expression | |
| Protein Expression | |
| Untagged |
| GS115, KM71 | |
| Restriction Enzyme⁄ MCS | |
| Yeast Cells | |
| P. pastoris | |
| Ampillicin | |
| Yeast | |
| Expression Kit | |
| 1 Kit |
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For Research Use Only. Not for use in diagnostic procedures.
