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  7. Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
  8. 10734227

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Brand:  Applied Biosystems™ 4391178

163.35 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10734227

  • 297.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

  • Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
  • Formulated to ensure maximum sensitivity and reliability
  • Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets

Includes:

  • AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
  • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
  • An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
  • RNase inhibitor, to prevent degradation of RNA templates
  • A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Sufficient for 40 reactions at 50 μL reaction volume and includes:
• 1 x 1 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening.
• 1 x 20 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C.


Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Tube
AmpliTaq Gold DNA Polymerase
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
SYBR Green
RNA-to-CT™, SYBR™
UP (Ultra Pure)
ArrayScript™ UP
Wet Ice
Gene Expression
40 Reactions
1 x 1 mL
SYBR
1-step RT-qPCR
Standard
Green RNA-to-CT 1-Step Kit
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
40 reactions
Standard
40 Reactions at 50 μL
2X (MasterMix), 125X (RT Enzyme Mix)
RNA
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For Research Use Only. Not for use in diagnostic procedures.