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Novus Biologicals™ INSTA-Blot Human Cell Lines
Applications: Western Blot
Brand: Novus Biologicals™ NBP2-30114
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Description
- Screening proteins from various human tissues
- Contains 11 human tissue lysates (20μg total protein per lane) on 4-20% Tris-Glycine mini gel
- Prepared from resolution by SDS-PAGE at 125V for 1 hour
- Proteins are electro-blotted for 2 hours at 25V onto PVDF membrane
- Stained with amido black for visualization of proteins
Recommended Storage
Store at room temperature
Specifications
| Human cell lines are lysed in 1X lysis buffer (10 mM Tris, pH 8.0,130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the cell lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).Use in Western blot reported in scientific literature (PMID: 24842606) | |
| 1 Membrane | |
| PVDF |
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