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Invitrogen™ AccuPrime™ Taq DNA Polymerase System
Descrizione
Includes
AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL); 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
Platinum anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.
AccuPrime Taq DNA Polymerase features
- Control mispriming at every cycle for single-band PCR products
- Minimal reaction optimization and primer set redesign
- Reaction assembly at room temperature
Applications
AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including:
- Multiplex PCR
- TOPO TA Cloning
- Allele-specific amplifications
- High-throughput screening
AccuPrime buffers
The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
Order Info
Shipping Condition: Approved for shipment on wet or dry ice
Specifica
Specifica
| Concentration | 10X |
| Content And Storage | • AccuPrime Taq DNA Polymerase (100 μL, at 2 U/μL) • 10X AccuPrime PCR Buffer I (500 μL) • 10X AccuPrime PCR Buffer II (500 μL) • 50 mM Magnesium Chloride (500 μL) Store at -20°C. |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | Low |
| PCR Method | 1-step RT-qPCR, Multiplex qPCR |
| Polymerase | AccuPrime Taq DNA Polymerase |
| Reaction Speed | Standard |
| Product Type | DNA Polymerase System |
| Quantity | 200 rxns |
| Shipping Condition | Wet or Dry Ice |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
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