Learn More
Invitrogen™ DNase I, Amplification Grade
Purified and tested for nondetectable levels of RNase contamination
Brand: Invitrogen™ 18068015
188.81 EUR valid until 2025-03-28
Use promo code "24107" to get your promotional price.
Includes: DNase I, Amp Grade (100U), 10X DNase I Reaction Buffer, 25mM EDTA (pH 8.0)
Description
DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.
- Specific activity is >10,000 units/mg
- Source: Purified from bovine pancreas
- Performance and quality testing: Ribonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined
- Unit definition: One unit increases absorbance of high molecular weight DNA solution at rate of 0.001 A260 units/min./mL of reaction mixture at 25°C
- Unit reaction conditions: 0.1M sodium acetate (pH 5.0), 5mM MgCl2, 50 μg/mL calf thymus DNA, and enzyme in 1mL for 10 min. at 25°C
Removing DNA from RNA and protein preparations, PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription
Order Info
Shipping Conditions: Approved for shipment on wet or dry ice

Specifications
Contents: 1 vial of DNase I, Amp Grade (100 U) 1 vial of 10X DNase I Reaction Buffer (1000 μL) 1 vial of 25 mM EDTA (pH 8.0) (200 μL) Store at -20°C in a non-frost-free freezer. |
|
DNase | |
100 U |
Approved for shipment on Wet or Dry Ice | |
Reaction Buffer |
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
3037373 | Certificate of Analysis | 31/01/2025 | 18068015 |
3137980 | Certificate of Analysis | 31/01/2025 | 18068015 |
2458208 | Certificate of Analysis | 29/01/2025 | 18068015 |
2476128 | Certificate of Analysis | 29/01/2025 | 18068015 |
2489131 | Certificate of Analysis | 29/01/2025 | 18068015 |
Promotions
-
Multiple Manufacturers
New Offer Shop Now
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.