Dynabeads MyOne Streptavidin C1 beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin. The product is intended for use in the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands. Their small but uniform size presents a high surface area per mg of beads and a correspondingly high capacity for the target ligand, while the high iron content ensures an efficient magnetic pull. These features, along with a slow sedimentation rate during incubation contribute to a gentle and rapid magnetic separation. The excellent dispersion abilities and the absence of magnetic remanence make the Dynabeads streptavidin products ideal for both manual and automated protocols, including microfluidic system protocols.
Benefits of Dynabeads MyOne Streptavidin C1 beads include: • Performance—high sensitivity and reproducibility secure consistent results • Efficiency-—ast binding kinetics and high binding capacity • Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics • Automation ready—easy automation for high-throughput needs on the Kingfisher Purification System
Consistency and reproducibility The monolayer of streptavidin leaves most biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results.
High binding capacity The size of the ligand and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands. With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads MyOne Streptavidin C1 typically binds: • >2,800 pmoles free biotin • ∼20 μg biotinylated IgG • ∼400 pmol biotinylated peptides • ∼20 μg ds-DNA • ∼500 pmol ss-oligonucleotides
Applications Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications. Although the beads can be used to isolate any biotinylated targets, these hydrophilic beads are particularly well suited for the preparation of ssDNA, high-throughput nucleic acid clean-up protocols, and protein purifications prior to mass spectrometry.
Automation ready with the KingFisher Purification System The 1-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers.
Commercial supply Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads MyOne Streptavidin C1 beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.
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