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Invitrogen™ One Shot™ ccdB Survival™ 2 T1^R Competent Cells

Brand: Invitrogen™ A10460

216.67 EUR valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 10733874

325.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

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Includes: ccdB Survival2 T1R Competent Cells: 11 vials, 50μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 1 bottle, 6mL

This item is not returnable. View return policy

Description

One Shot ccdB Survival 2 T1^R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors. ccdB Survival 2 T1R cells that are made chemically competent can achieve high transformation efficiency of >1 x 10⁹ transformants/μg plasmid DNA.

The ccdB Survival 2 T1^R E. coli strain is derived from the TOP10 strain and offers the same benefits. Mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allow cloning of both prokaryotic and eukaryotic genomic DNA that contains methylcytosine and methyladenine. This strain also has the lacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. In addition, recA1 and endA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps.The tonA (also known as fhuA) mutation in ccdB Survival 2 T1^R cells confers resistance to bacteriophages T1 and T5, safeguarding samples against contamination.

ccdB Survival 2 T1^R Competent Cells offer:
• >1 x 10⁹ transformants/μg efficiency
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Support of high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)

Convenient and efficient format
One Shot ccdB Survival 2 T1^R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells.

Genotype: F^-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL(Str^R) endA1 nupG fhuA::IS2

Find the strain and format that fits your needs
• We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
• MultiShot formats for high throughput applications are available.

Order Info

Shipping Condition: Dry Ice

Specifications

Product Type	Chemically Competent Cells
Improves Plasmid Quality	Yes (endA1)
Transformation Efficiency Level	High Efficiency (>1 x 10⁹ cfu/μg)
Antibiotic Resistance Bacterial	Yes (Streptomycin)
Blue/White Screening	Yes (lacZΔM15)
Plasmid	ccdB vector propagation
Reduces Recombination	Yes (recA1)
T1 Phage - Resistant (tonA)	Yes
Format	Tube
Quantity	11 x 50 μL

Contains F' Episome	No
Cloning Methylated DNA	Yes (mcrA)
Content And Storage	• One Shot ccdB Survival 2 T1R Competent Cells (11 x 50 μL) Store Competent Cells at &ndash80°C. • pUC19 DNA (50 μL at 10 pg/μL) Store pUC19 DNA at –20°C. • S.O.C. Medium (6 ml) Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA	Not suitable for cloning unstable DNA
High-throughput Compatibility	Low
Preparing Unmethylated DNA	No
Shipping Condition	Dry Ice
Species	E. coli (K12)
Product Line	One Shot™

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Documents

Product Information

Manuals

One Shot ccdB Survival 2 T1R Chemically Competent Cells Version: 2.00 File size: 187 kB

Bacterial Cells- Statement of Nonpathogenicity File size: 425.2 kB

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number2490504	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA10460
Lot Number2534760A	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA10460
Lot Number2562871	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA10460
Lot Number2602337A	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA10460
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Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells