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Invitrogen™ pIZ/V5-His Vector Kit

For high-level expression in a variety of insect cells

Brand:  Invitrogen™ V800001

Product Code. 10674383

  • 1102.00 € / 1 set

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For Research Use Only. All usage must comply with product instructions.


Includes: The InsectSelect Kit includes 20μg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2μg of lyophilized OpIE2 Reverse Sequencing primer, 1g Zeocin, Cellfectin Reagent, and 1L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO Grace's Insect Media, High Five Cells are supplied with GIBCO Express Five Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above.
This item is not returnable. View return policy

Beschreibung

Beschreibung

  • This exceptionally small vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells
  • The OpIE2 promoter for constitutive expression
  • The Zeocin resistance gene for rapid selection of stably transfected cell lines
  • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin

Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY
Spezifikation

Spezifikation

OplE2
The InsectSelect™ Kit includes 20 μg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2 μg of lyophilized OpIE2 Reverse Sequencing primer, 1 g Zeocin™, Cellfectin™ Reagent, and 1 L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO™ Grace's Insect Media, High Five™ Cells are supplied with GIBCO™ Express Five™ Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
His Tag (6x), V5 Epitope Tag
1 kit
InsectSelect™
Insect Cell Expression Vector
C-terminal
Restriction Enzyme/MCS
pIZ
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Product Information

Manuals

InsectSelect System with pIZ/V5-His Version: 250282 Version H; 21 October 2010 File size: 408.7 kB
Zertifikate
Sonderangebote

Sonderangebote

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Invitrogen™ pIZ/V5-His Vector Kit > 1 kit

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For Research Use Only. Not for use in diagnostic procedures.