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  7. Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
  8. 10499763

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

Brand:  Invitrogen™ 11754050

576.80 EUR valid until 2024-12-31
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Product Code. 10499763

  • 824.00 € / 50 reactions

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For Research Use Only. All usage must comply with product instructions.


Includes: 5X VILO Reaction Mix (200μL) and a 10X SuperScript III Enzyme Blend (100μL), which contains: SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein
This item is not returnable. View return policy

Description

Description

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat. No. 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat. No. 11766050).
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 5X VILO Reaction Mix, 200 μL
• 10X SuperScript III Enzyme Blend, 100 μL

Store at –5 to –30°C.
Kit
60 min.
42°C
SuperScript III
Dry Ice
First-Strand cDNA
Master Mix
Up to 10 kb
qPCR
High
Reverse Transcription
50 reactions
Reduced
Real Time PCR (qPCR)
50 Reactions
Reverse Transcription
RNA
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For Research Use Only. Not for use in diagnostic procedures.