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Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

Brand: Invitrogen™ 11754250

Product Code. 10704274

3695.00 € / 250 reactions

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For Research Use Only. All usage must comply with product instructions.

Includes: 5X VILO Reaction Mix (200μL) and a 10X SuperScript III Enzyme Blend (100μL), which contains: SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein

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Description

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat. No. 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat. No. 11766050).
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

Order Info

Shipping Condition: Dry ice

Specifications

Content And Storage	• 5X VILO Reaction Mix, 1,000 μL • 10X SuperScript III Enzyme Blend, 500 μL Store at –5 to –30°C.
Format	Kit
Reaction Speed	60 min.
Optimal Reaction Temperature	42°C
Reverse Transcriptase	SuperScript III
Shipping Condition	Dry Ice
Final Product Type	First-Strand cDNA
Reaction Format	Master Mix
Size (Final Product)	Up to 10 kb

Detection Method	qPCR
GC-Rich PCR Performance	High
Technique	Reverse Transcription
Quantity	250 reactions
Ribonuclease H Activity	Reduced
For Use With (Application)	Real Time PCR (qPCR)
No. of Reactions	250 Reactions
Reagent Type	Reverse Transcription
Starting Material	RNA

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Documents

Product Information

Manuals

Product Info Sheet: SuperScript VILO cDNA Synthesis Kit (Japanese) Version: 08January2008 File size: 125.8 kB

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number3006819	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number11754250_LITL18 PROMO,11754250
Lot Number2449650	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number11754250_LITL18 PROMO,11754250
Lot Number2468297	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number11754250_LITL18 PROMO,11754250
Lot Number2800352	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number11754250_LITL18 PROMO,11754250
Lot Number2867316	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog Number11754250_LITL18 PROMO,11754250

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