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Thermo Scientific™ LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, goat
The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of ligand-dependent coactivator displacement.
Brand: Thermo Scientific™ PV4836
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Description
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15141:
The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:
When running the LanthaScreen™ TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand's EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).
Specifications
| Tb (Terbium) | |
| Fluorescence | |
| CAR-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, terbium-labeled anti-GST antibody, and buffers | |
| For research use only (RUO) | |
| The LanthaScreen™ TR-FRET CAR Coactivator Assay Kit contains CAR-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C). |
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| End Point | |
| CAR, NR1I3 | |
| Co-Factor Interaction Assay, TR-FRET | |
| TR-FRET CAR Coactivator Assay Kit |
| LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation | |
| Microplate Reader | |
| 800 x 20 μL Assays | |
| 384-well Plate | |
| Biochemical coregulator interaction | |
| Dry Ice | |
| LanthaScreen™ | |
| CAR | |
| 800 x 20 μL Assays |
For Research Use Only. Not for use in diagnostic procedures.