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Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Red/Blue is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

Brand:  Invitrogen™ R37610

262.20 EUR valid until 2025-03-28
Use promo code "24107" to get your promotional price.

Product Code. 15450054

  • 345.00 € / 1 set

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For Research Use Only. All usage must comply with product instructions.


Includes: 3 x 2.5mL NucBlue Live, 3 x 2.5mL propidium iodide
This item is not returnable. View return policy

Description

Description

ReadyProbes™ Cell Viability Imaging Kit, Red/Blue contains NucBlue™ Live reagent (Hoechst 33342) and propidium iodide. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set.

  • NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
  • Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617nm)

Suggestions for use

  • NucBlue™ Live reagent and propidium iodide can be added directly to cells in full growth media or a compatible buffer solution.
  • In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Mammalian Cells, Eukaryotic Cells
Store at 2° to 8°C, or at room temperature.
Fluorescence
Fluorescence Microscope
3 x 2.5 mL NucBlue™ Live; 3 x 2.5 mL propidium iodide
360, 535 nm
ReadyProbes
Room Temperature
Blue, Red
ReadyProbes Cell Viability Imaging Kit, Blue/Red
Viability Assay
Cell Viability Imaging Kit
460, 617 nm
Dropper bottles
1 kit
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For Research Use Only. Not for use in diagnostic procedures.